Differential Display Methods and Protocols

Drs. Peng Liang and Arthur B. Pardee have in Differential Display Methods and Protocols assembled for the first time a comprehensive review of the state of the art of their powerful new methodology and its practical applications. The book's pioneering contributors describe all the major element...

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Bibliographic Details
Other Authors: Liang, Peng (Editor), Pardee, Arthur B. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 1997, 1997
Edition:1st ed. 1997
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • Identification of Immediate—Early Gene Targets of the Raf-1 Serine/Threonine Protein Kinase Using an Estradiol-Dependent Fusion Protein, ?Raf-1:ER
  • Isolation of Cytokine-Inducible Genes from Hematopoietic Cells by Differential Display
  • Hormone-lnducible Genes in Prostate Cells
  • Cloning Differentially Expressed Genes During Normal Development
  • Application of Differential Display in Studying Developmental Processes
  • Identification of Novel Genes Involved in Adipose Differentiation by Differential Display
  • Isolation of Song-Regulated Genes in the Brain of Songbirds
  • Identification of Vertebrate Circadian Clock-Regulated Genes by Differential Display
  • Cloning Differentially Expressed Genes in Diseases
  • Differential mRNA Display
  • Differential Expression of TIMP-3 During Neoplastic Progression in the Mouse JB6 Model System
  • Identification of Genes Associated with Resistance to Photodynamic Therapy-Mediated Oxidative Stress
  • Methodologies
  • Differential Display
  • Fingerprinting by Arbitrarily Primed PCR
  • Differential Display Using Random Hexamer-Primed cDNA, Motif Primers, and Agarose Gel Electrophoresis
  • Fluorescent Differential Display
  • Cloning Differentially Expressed Genes by Using Differential Display and Subtractive Hybridization
  • Direct Sequencing of Differential Display PCR Products
  • A Direct-Sequencing-Based Strategy for Identifying and Cloning cDNAs from Differential Display Gels
  • Differential Screening of Differential Display cDNA Products by Reverse Northern
  • Screening for Positive Clones Generated by Differential Display
  • Cloning Family-Specific Genes
  • Cloning of the 3? Noncoding Regions from Several Members of Heat Shock Protein Gene Families by Differential Display
  • RC4D—Restriction Fragment Length Polymorphism-Coupled Domain-Directed Differential Display
  • Cloning Inducible Genes
  • RAP-PCR Using RNA from Tissue Microdissection
  • The Application of Differential Display to the Brain
  • Analysis of Gene Expression in Hypothalamus in Obese and Normal Mice Using Differential Display