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090727 ||| eng |
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|a 9781592595693
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| 050 |
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4 |
|a QH573-671
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| 100 |
1 |
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|a Liang, Peng
|e [editor]
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| 245 |
0 |
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|a Differential Display Methods and Protocols
|h Elektronische Ressource
|c edited by Peng Liang, Arthur B. Pardee
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| 250 |
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|a 1st ed. 1997
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| 260 |
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|a Totowa, NJ
|b Humana Press
|c 1997, 1997
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| 300 |
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|a XIV, 306 p
|b online resource
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| 505 |
0 |
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|a Identification of Immediate—Early Gene Targets of the Raf-1 Serine/Threonine Protein Kinase Using an Estradiol-Dependent Fusion Protein, ?Raf-1:ER -- Isolation of Cytokine-Inducible Genes from Hematopoietic Cells by Differential Display -- Hormone-lnducible Genes in Prostate Cells -- Cloning Differentially Expressed Genes During Normal Development -- Application of Differential Display in Studying Developmental Processes -- Identification of Novel Genes Involved in Adipose Differentiation by Differential Display -- Isolation of Song-Regulated Genes in the Brain of Songbirds -- Identification of Vertebrate Circadian Clock-Regulated Genes by Differential Display -- Cloning Differentially Expressed Genes in Diseases -- Differential mRNA Display -- Differential Expression of TIMP-3 During Neoplastic Progression in the Mouse JB6 Model System -- Identification of Genes Associated with Resistance to Photodynamic Therapy-Mediated Oxidative Stress --
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| 505 |
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|a Methodologies -- Differential Display -- Fingerprinting by Arbitrarily Primed PCR -- Differential Display Using Random Hexamer-Primed cDNA, Motif Primers, and Agarose Gel Electrophoresis -- Fluorescent Differential Display -- Cloning Differentially Expressed Genes by Using Differential Display and Subtractive Hybridization -- Direct Sequencing of Differential Display PCR Products -- A Direct-Sequencing-Based Strategy for Identifying and Cloning cDNAs from Differential Display Gels -- Differential Screening of Differential Display cDNA Products by Reverse Northern -- Screening for Positive Clones Generated by Differential Display -- Cloning Family-Specific Genes -- Cloning of the 3? Noncoding Regions from Several Members of Heat Shock Protein Gene Families by Differential Display -- RC4D—Restriction Fragment Length Polymorphism-Coupled Domain-Directed Differential Display -- Cloning Inducible Genes --
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| 505 |
0 |
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|a RAP-PCR Using RNA from Tissue Microdissection -- The Application of Differential Display to the Brain -- Analysis of Gene Expression in Hypothalamus in Obese and Normal Mice Using Differential Display
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| 653 |
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|a Cell Biology
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| 653 |
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|a Cytology
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| 700 |
1 |
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|a Pardee, Arthur B.
|e [editor]
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| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SPRPROT
|a Springer Protocols Archive 1981-2004
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| 490 |
0 |
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|a Methods in Molecular Biology
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| 024 |
8 |
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|a 10.1385/0896034895
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| 856 |
4 |
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|u https://doi.org/10.1385/0896034895?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 571.6
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| 520 |
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|a Drs. Peng Liang and Arthur B. Pardee have in Differential Display Methods and Protocols assembled for the first time a comprehensive review of the state of the art of their powerful new methodology and its practical applications. The book's pioneering contributors describe all the major elements of this novel technology, including both RAP-PCR and DD using fluorescence detection, as well as their powerful strategy for identifying and cloning family-specific genes. Also provided are numerous examples-along with detailed experimental procedures-in which differentially expressed genes have been successfully identified in diverse biological systems ranging from plants to songbirds to humans. Comprehensive and on the cutting-edge, Differential Display Methods and Protocols provides readers with precise new tools for studying how gene expression is exactly regulated throughout the development of a living organism, and how the failure of this intricate control mechanism leads to pathological complications. This novel and productive methodology, fully detailed here, is already playing a major role in the development of selective antagonists and inhibitors for treating cancer, cardiovascular disease, CNS disorders, and inflammation and tissue repair. Now scientists everywhere can successfully apply this powerful set of techniques using the step-by-step procedures of its innovators
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