Basic DNA and RNA Protocols
| Other Authors: | |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
1996, 1996
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| Edition: | 1st ed. 1996 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Terminal Labeling of DNA for Maxam and Gilbert Sequencing
- DNA Sequencing by the Chemical Method
- Site-Directed Mutagenesis and Protein Synthesis
- Site-Directed Mutagenesis of Double-Stranded Plasmids, Domain Substitution, and Marker Rescue by Comutagenesis of Restriction Enzyme Sites
- A Protocol for Site-Directed Mutagenesis Employing a Uracil-Containing Phagemid Template
- In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System
- In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System
- One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin
- Polymerase Chain Reaction
- Inverse Polymerase Chain Reaction
- Polymerase Chain Reaction with Degenerate Oligonucleotide Primers to Clone Gene Family Members
- Cloning PCR Products Using T-Vectors
- Direct Radioactive Labeling of Polymerase Chain Reaction Products
- A Rapid PCR-Based Colony Screening Protocol for Cloned Inserts
- Use of Polymerase Chain Reaction to Screen Phage Libraries
- DNA Sequencing
- Cloning into M13 Bacteriophage Vectors
- Ordered Deletions Using Exonuclease III
- M13 Phage Growth and Single-Stranded DNA Preparation
- Preparation of ssDNA from Phagemid Vectors
- A Rapid Plasmid Purification Method for Dideoxy Sequencing
- DNA Sequencing Using Sequenase Version 2.0 T7 DNA Polymerase
- Pouring Linear and Buffer-Gradient Sequencing Gels
- Electrophoresis of Sequence Reaction Samples
- Direct Sequencing of PCR Products
- Thermal Cycle Dideoxy DNA Sequencing
- Using the Automated DNA Sequencer
- DNA Analysis
- The Simultaneous Isolation of RNA and DNA from Tissues and Cultured Cells
- Restriction Endonuclease Digestion of DNA
- Agarose Gel Electrophoresis
- Capillary Blotting of Agarose Gels
- Random Primed 32P-Labeling of DNA
- Hybridization and Competition Hybridization of Southern Blots
- Utilization of DNA Probes with Digoxigenin-Modified Nucleotides in Southern Hybridizations
- The Preparation of Fluorescein-Labeled Nucleic Acid Probes and Their Detection Using Alkaline Phosphatase-Catalyzed Dioxetane Chemiluminescence
- Monitoring Incorporation of Fluorescein into Nucleic Acid Probes Using a Rapid Labeling Assay
- The Preparation of Horseradish Peroxidase Labeled Nucleic Acid Probes and Their Detection Using Enhanced Chemiluminescence
- 3?-End Labeling of Oligonucleotides with Fluorescein-11-dUTP and Enhanced Chemiluminescent Detection
- The Preparation of Riboprobes
- Native Polyacrylamide Gel Electrophoresis
- Use of Silver Staining to Detect Nucleic Acids
- End-Labeling of DNA Fragments
- RNA Analysis
- Northern Blot Analysis
- RNA Slot Blotting
- The RNase Protection Assay
- Primer Extension Analysis of mRNA
- S1 Mapping Using Single-Stranded DNA Probes
- Nonradioactive In Situ Hybridization for Cells and Tissues
- Gene Cloning
- In Vitro Packaging of DNA
- Construction of Mammalian Genomic Libraries Using ? Replacement Vectors
- The Production of Double-Stranded Complementary DNA for Use in Making Libraries
- Construction of cDNA Libraries
- Screening ? Libraries
- Subcloning Methods
- Subcloning Strategies and Protocols
- Purification of DNA Fragments from Agarose Gels Using Glass Beads
- Transformation of E. coli
- Transformation of Bacteria by Electroporation
- Preparation of Plasmid DNA by Alkaline Lysis
- The Rapid Boiling Method for Small-Scale Preparation of Plasmid DNA
- Plasmid Preparations with Diatomaceous Earth
- PCR Techniques