|
|
|
|
| LEADER |
04441nmm a2200313 u 4500 |
| 001 |
EB000285595 |
| 003 |
EBX01000000000000000049710 |
| 005 |
00000000000000.0 |
| 007 |
cr||||||||||||||||||||| |
| 008 |
090727 ||| eng |
| 020 |
|
|
|a 9781592592517
|
| 050 |
|
4 |
|a QH573-671
|
| 100 |
1 |
|
|a Harwood, Adrian J.
|e [editor]
|
| 245 |
0 |
0 |
|a Basic DNA and RNA Protocols
|h Elektronische Ressource
|c edited by Adrian J. Harwood
|
| 250 |
|
|
|a 1st ed. 1996
|
| 260 |
|
|
|a Totowa, NJ
|b Humana Press
|c 1996, 1996
|
| 300 |
|
|
|a 528 p
|b online resource
|
| 505 |
0 |
|
|a Terminal Labeling of DNA for Maxam and Gilbert Sequencing -- DNA Sequencing by the Chemical Method -- Site-Directed Mutagenesis and Protein Synthesis -- Site-Directed Mutagenesis of Double-Stranded Plasmids, Domain Substitution, and Marker Rescue by Comutagenesis of Restriction Enzyme Sites -- A Protocol for Site-Directed Mutagenesis Employing a Uracil-Containing Phagemid Template -- In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System -- In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System -- One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin
|
| 505 |
0 |
|
|a Polymerase Chain Reaction -- Inverse Polymerase Chain Reaction -- Polymerase Chain Reaction with Degenerate Oligonucleotide Primers to Clone Gene Family Members -- Cloning PCR Products Using T-Vectors -- Direct Radioactive Labeling of Polymerase Chain Reaction Products -- A Rapid PCR-Based Colony Screening Protocol for Cloned Inserts -- Use of Polymerase Chain Reaction to Screen Phage Libraries -- DNA Sequencing -- Cloning into M13 Bacteriophage Vectors -- Ordered Deletions Using Exonuclease III -- M13 Phage Growth and Single-Stranded DNA Preparation -- Preparation of ssDNA from Phagemid Vectors -- A Rapid Plasmid Purification Method for Dideoxy Sequencing -- DNA Sequencing Using Sequenase Version 2.0 T7 DNA Polymerase -- Pouring Linear and Buffer-Gradient Sequencing Gels -- Electrophoresis of Sequence Reaction Samples -- Direct Sequencing of PCR Products -- Thermal Cycle Dideoxy DNA Sequencing -- Using the Automated DNA Sequencer --
|
| 505 |
0 |
|
|a DNA Analysis -- The Simultaneous Isolation of RNA and DNA from Tissues and Cultured Cells -- Restriction Endonuclease Digestion of DNA -- Agarose Gel Electrophoresis -- Capillary Blotting of Agarose Gels -- Random Primed 32P-Labeling of DNA -- Hybridization and Competition Hybridization of Southern Blots -- Utilization of DNA Probes with Digoxigenin-Modified Nucleotides in Southern Hybridizations -- The Preparation of Fluorescein-Labeled Nucleic Acid Probes and Their Detection Using Alkaline Phosphatase-Catalyzed Dioxetane Chemiluminescence -- Monitoring Incorporation of Fluorescein into Nucleic Acid Probes Using a Rapid Labeling Assay -- The Preparation of Horseradish Peroxidase Labeled Nucleic Acid Probes and Their Detection Using Enhanced Chemiluminescence -- 3?-End Labeling of Oligonucleotides with Fluorescein-11-dUTP and Enhanced Chemiluminescent Detection -- The Preparation of Riboprobes -- Native Polyacrylamide Gel Electrophoresis --
|
| 505 |
0 |
|
|a Use of Silver Staining to Detect Nucleic Acids -- End-Labeling of DNA Fragments -- RNA Analysis -- Northern Blot Analysis -- RNA Slot Blotting -- The RNase Protection Assay -- Primer Extension Analysis of mRNA -- S1 Mapping Using Single-Stranded DNA Probes -- Nonradioactive In Situ Hybridization for Cells and Tissues -- Gene Cloning -- In Vitro Packaging of DNA -- Construction of Mammalian Genomic Libraries Using ? Replacement Vectors -- The Production of Double-Stranded Complementary DNA for Use in Making Libraries -- Construction of cDNA Libraries -- Screening ? Libraries -- Subcloning Methods -- Subcloning Strategies and Protocols -- Purification of DNA Fragments from Agarose Gels Using Glass Beads -- Transformation of E. coli -- Transformation of Bacteria by Electroporation -- Preparation of Plasmid DNA by Alkaline Lysis -- The Rapid Boiling Method for Small-Scale Preparation of Plasmid DNA -- Plasmid Preparations with Diatomaceous Earth -- PCR Techniques --
|
| 653 |
|
|
|a Cell Biology
|
| 653 |
|
|
|a Cytology
|
| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
|
| 989 |
|
|
|b SPRPROT
|a Springer Protocols Archive 1981-2004
|
| 490 |
0 |
|
|a Methods in Molecular Biology
|
| 024 |
8 |
|
|a 10.1385/089603402X
|
| 856 |
4 |
0 |
|u https://doi.org/10.1385/089603402X?nosfx=y
|x Verlag
|3 Volltext
|
| 082 |
0 |
|
|a 571.6
|