Basic Protein and Peptide Protocols
| Other Authors: | |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
1994, 1994
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| Edition: | 1st ed. 1994 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Peptide Purification by Reverse-Phase HPLC
- Enzymatic Methods for Cleaving Proteins
- Chemical Cleavage of Proteins
- Chemical Modification of Proteins
- The Dansyl Method for Identifying N-Terminal Amino Acids
- The Dansyl-Edman Method for Peptide Sequencing
- C-Terminal Sequencing of Peptides
- Analysis of Cysteine Residues and Disulfide Bonds
- Preparation and Characterization of Monoclonal Antibodies to Proteins and Other Cellular Components
- The Raising of a Polyclonal Antiserum to a Protein
- Production of Antisera to Synthetic Peptides
- Production of Antibodies Using Proteins in Gel Bands
- Preparation of Purified Immunoglobulin G (IgG)
- Enzyme and Biotin Labeling of Antibody
- Labeling of Peptides and Proteins by Radioiodination
- Radioimmunoassay of Peptides and Proteins
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Free Zone Capillary Electrophoresis
- The Lowry Method for Protein Quantitation
- The Bicinchoninic Acid (BCA) Assay for Protein Quantitation
- The Bradford Method for Protein Quantitation
- Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins
- SDS Polyacrylamide Gel Electrophoresis of Proteins
- Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins
- Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Proteins
- Electrophoresis of Peptides
- Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels
- The In Vivo Isotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis
- Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins
- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Immobilized pH Gradients in the First Dimension
- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension
- Quantification of Proteins on Polyacrylamide Gels (Nonradioactive)
- Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique
- Identification of Glycoproteins on Nitrocellulose Membranes and Gels
- The Release of Oligosaccharides from Glycoproteins
- Structural Profiling of Oligosaccharides of Glycoproteins
- Drying Gels
- Fluorography of Polyacrylamide Gels Containing Tritium
- The Electrophoretic Elution of Proteins from Polyacrylamide Gels
- Preparation of Proteins from Gels for Protein Microsequencing
- Comparison of Protein Primary Structures
- Protein Blotting
- Detection of Polypeptides on Immunoblots Using Secondary Antibodies or Protein A
- Detection of Proteins on Blots Using the Avidin-Biotin System
- Detection of Proteins on Protein Blots Using Chemiluminescent Systems
- The Detection of Proteins on Blots Using Gold or Immunogold
- Amino Acid Analysis
- Molecular-Weight Estimation for Native Proteins Using Size-Exclusion High-Performance Liquid Chromatography