|
|
|
|
| LEADER |
03697nmm a2200289 u 4500 |
| 001 |
EB000285591 |
| 003 |
EBX01000000000000000049706 |
| 005 |
00000000000000.0 |
| 007 |
cr||||||||||||||||||||| |
| 008 |
090727 ||| eng |
| 020 |
|
|
|a 9781592595198
|
| 050 |
|
4 |
|a QD415-436
|
| 100 |
1 |
|
|a Walker, John M.
|e [editor]
|
| 245 |
0 |
0 |
|a Basic Protein and Peptide Protocols
|h Elektronische Ressource
|c edited by John M. Walker
|
| 250 |
|
|
|a 1st ed. 1994
|
| 260 |
|
|
|a Totowa, NJ
|b Humana Press
|c 1994, 1994
|
| 300 |
|
|
|a XII, 490 p
|b online resource
|
| 505 |
0 |
|
|a Peptide Purification by Reverse-Phase HPLC -- Enzymatic Methods for Cleaving Proteins -- Chemical Cleavage of Proteins -- Chemical Modification of Proteins -- The Dansyl Method for Identifying N-Terminal Amino Acids -- The Dansyl-Edman Method for Peptide Sequencing -- C-Terminal Sequencing of Peptides -- Analysis of Cysteine Residues and Disulfide Bonds -- Preparation and Characterization of Monoclonal Antibodies to Proteins and Other Cellular Components -- The Raising of a Polyclonal Antiserum to a Protein -- Production of Antisera to Synthetic Peptides -- Production of Antibodies Using Proteins in Gel Bands -- Preparation of Purified Immunoglobulin G (IgG) -- Enzyme and Biotin Labeling of Antibody -- Labeling of Peptides and Proteins by Radioiodination -- Radioimmunoassay of Peptides and Proteins -- Enzyme-Linked Immunosorbent Assay (ELISA) -- Free Zone Capillary Electrophoresis
|
| 505 |
0 |
|
|a The Lowry Method for Protein Quantitation -- The Bicinchoninic Acid (BCA) Assay for Protein Quantitation -- The Bradford Method for Protein Quantitation -- Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins -- SDS Polyacrylamide Gel Electrophoresis of Proteins -- Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins -- Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Proteins -- Electrophoresis of Peptides -- Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels -- The In Vivo Isotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis -- Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins -- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Immobilized pH Gradients in the First Dimension -- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension -- Quantification of Proteins on Polyacrylamide Gels (Nonradioactive) --
|
| 505 |
0 |
|
|a Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique -- Identification of Glycoproteins on Nitrocellulose Membranes and Gels -- The Release of Oligosaccharides from Glycoproteins -- Structural Profiling of Oligosaccharides of Glycoproteins -- Drying Gels -- Fluorography of Polyacrylamide Gels Containing Tritium -- The Electrophoretic Elution of Proteins from Polyacrylamide Gels -- Preparation of Proteins from Gels for Protein Microsequencing -- Comparison of Protein Primary Structures -- Protein Blotting -- Detection of Polypeptides on Immunoblots Using Secondary Antibodies or Protein A -- Detection of Proteins on Blots Using the Avidin-Biotin System -- Detection of Proteins on Protein Blots Using Chemiluminescent Systems -- The Detection of Proteins on Blots Using Gold or Immunogold -- Amino Acid Analysis -- Molecular-Weight Estimation for Native Proteins Using Size-Exclusion High-Performance Liquid Chromatography --
|
| 653 |
|
|
|a Biochemistry
|
| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
|
| 989 |
|
|
|b SPRPROT
|a Springer Protocols Archive 1981-2004
|
| 490 |
0 |
|
|a Methods in Molecular Biology
|
| 024 |
8 |
|
|a 10.1385/089603268X
|
| 856 |
4 |
0 |
|u https://doi.org/10.1385/089603268X?nosfx=y
|x Verlag
|3 Volltext
|
| 082 |
0 |
|
|a 572
|