Transmembrane β-Barrel Proteins Methods and Protocols
This detailed volume explores experimental strategies and protocols for the expression, assembly, characterization, and exploitation of transmembrane β-barrel proteins. Beginning with methodologies to study their assembly, the book continues with protocols for characterizing the landscape of transme...
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| Format: | eBook |
| Language: | English |
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New York, NY
Humana
2024, 2024
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| Edition: | 1st ed. 2024 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- The Name is Barrel, β-Barrel
- Recombinant Expression and Overproduction of Transmembrane b-Barrel Proteins
- Bacterial Envelope Fractionation
- Fluorescent Labeling of Outer Membrane Proteins Using the SpyCatcher-SpyTag System
- In Vitro Reconstruction of Bacterial β-Barrel Membrane Protein Assembly Using E. coli Microsomal (Mid-Density) Membrane
- Examining Protein Translocation by β-Barrel Membrane Proteins Using Reconstituted Proteoliposomes
- In Vivo Disulfide-Bond Crosslinking to Study β-Barrel Membrane Protein Interactions, Dynamicity, and Folding Intermediates
- Site-Specific Photocrosslinking to Investigate Toxin Delivery Mediated by the Bacterial β-Barrel Assembly Machine
- Analysis of Transmembrane β-Barrel Proteins by Native and Semi-Native Polyacrylamide Gel Electrophoresis
- Affinity Purification of Membrane β-Barrel Proteins via Biotin-Tagged Peptidiscs
- Structural Modeling of T9SS Outer Membrane Proteins and Their Complexes
- Rationale in Custom Design of Transmembrane β-Barrel Pores
- Stress-Based Screening for Compounds that Inhibit β-Barrel Outer Membrane Protein Assembly in Gram-Negative Bacteria
- Monitoring the Interaction of the Peptidoglycan with the Bacterial β-Barrel Assembly Machinery
- MitoLuc:A Luminescence-Based Assay to Study Real-Time Protein Import into Mitochondria
- Modular Assembly of Mitochondrial β-Barrel Proteins
- Tracking the Activity and Position of Mitochondrial β-Barrel Proteins
- Conformational Heterogeneity of β-Barrel Membrane Proteins Observed In Situ Using Orthogonal Spin Labels and Pulsed ESR Spectroscopy
- Characterization of β-Barrel Outer Membrane Proteins and Their Interactions with Chaperones by Chemical-Crosslinking Mass Spectrometry
- Dissecting the Organization of a β-Barrel Assembly Machinery (BAM) Complex by Neutron Reflectometry
- Expression, Purification, and Cryo-EM Structural Analysis of an Outer Membrane Secretin Channel
- Recent Advances in Modeling Membrane β-Barrel Proteins Using Molecular Dynamics Simulations: From Their Lipid Environments to Their Assemblies