Mammalian Synthetic Systems
This volume details cutting-edge methods that enables engineering of novel functions in mammalian cells. Chapters guide readers through cell-to-cell communication, cell fate control, protein and RNA-based biosensors together with tools for more reliable and faster mammalian genome editing. Written i...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2024, 2024
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| Edition: | 1st ed. 2024 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- A directed evolution protocol for engineering minimal transcription factors, based on CIS display
- Set-up and applications of Modular Protein Expression Toolboxes (MoPET) for mammalian systems
- Coiled-coiled interaction toolbox for engineering mammalian cells
- A mammalian-based synthetic biology toolbox to engineer membrane-membrane interfaces
- Engineered protease-responsive RNA Binding Proteins (RBPs) to expand the toolbox of synthetic circuits in mammalian cells
- Mechanistic model-driven biodesign in mammalian synthetic biology
- Realizing Antithetic Integral Feedback Control in Mammalian Cells
- A computational modeling approach for the design of genetic control systems that respond to transcriptional activity
- Flux Balance Analysis of Mammalian Cell Systems
- Multiplex functional characterization of protein variant libraries in mammalian cells with single-copy genomic integration and high throughput DNA sequencing
- Flow Cytometry Quantification of Transient Transfections in Mammalian CellsRNA switches using Cas proteins
- Multiplexed transactivation of mammalian cells using dFnCas12a-VPR
- Anti-CRISPR Proteins and their Application to Control CRISPR-effectors in Mammalian Systems
- Post-translational Remote Control Mediated by Physical Inducers for Rapid Protein Release in Engineered Mammalian Cells
- Detection of microRNAs Using Synthetic Toehold Switch in Mammalian Cells
- Detection of microRNAs Using Synthetic Toehold Switch in Mammalian Cells
- High-throughput spectroscopic analysis of mRNA capping level
- In vitro generation of megakaryocytes from engineered mouse embryonic stem cells
- Preparation of chromobodies for the detection of cell surface epitopes