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090727 ||| eng |
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|a 9781592593842
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|a QH573-671
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| 100 |
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|a Bartlett, John M. S.
|e [editor]
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| 245 |
0 |
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|a PCR Protocols
|h Elektronische Ressource
|c edited by John M. S. Bartlett, David Stirling
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| 250 |
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|a 2nd ed. 2003
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| 260 |
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|a Totowa, NJ
|b Humana Press
|c 2003, 2003
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| 300 |
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|a 556 p
|b online resource
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| 505 |
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|a to PCR -- A Short History of the Polymerase Chain Reaction -- PCR Patent Issues -- Equipping and Establishing a PCR Laboratory -- Quality Control in PCR -- Preparation of Nucleic Acid Templates -- Extraction of Nucleic Acid Templates -- Extraction of DNA from Whole Blood -- DNA Extraction from Tissue -- Extraction of DNA from Microdissected Archival Tissues -- RNA Extraction from Blood -- RNA Extraction from Frozen Tissue -- RNA Extraction from Tissue Sections -- Dual DNA/RNA Extraction -- DNA Extraction from Fungi, Yeast, and Bacteria -- Isolation of RNA Viruses from Biological Materials -- Extraction of Ancient DNA -- DNA Extraction from Plasma and Serum -- Technical Notes for the Detection of Nucleic Acids -- Technical Notes for the Recovery and Purification of PCR Products from Acrylamide Gels -- Basic PCR Methods -- PCR Primer Design -- Optimization of Polymerase Chain Reactions --
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|a Subcycling PCR for Long-Distance Amplifications of Regions with High and Low Guanine-Cystine Content -- Rapid Amplification of cDNA Ends -- Randomly Amplified Polymorphic DNA Fingerprinting -- Microsphere-Based Single Nucleotide Polymorphism Genotyping -- Ligase Chain Reaction -- Nested RT-PCR in a Single Closed Tube -- Direct PCR from Serum -- Long PCR Amplification of Large Fragments of Viral Genomes -- Long PCR Methodology -- Ultrasensitive and Quantitative PCR -- Qualitative and Quantitative PCR -- Ultrasensitive PCR Detection of Tumor Cells in Myeloma -- Ultrasensitive Quantitative PCR to Detect RNA Viruses -- Quantitative PCR for cAMP RI Alpha mRNA -- Quantitation of Multiple RNA Species -- Transcriptome Analysis -- Differential Display -- AU-Differential Display, Reproducibility of a Differential mRNA Display Targeted to AU Motifs -- PCR Fluorescence Differential Display -- Microarray Analysis Using RNA Arbitrarily Primed PCR -- Oligonucleotide Arrays for Genotyping --
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| 505 |
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|a Serial Analysis of Gene Expression -- Mutations and Polymorphisms -- Mutation and Polymorphism Detection -- Combining Multiplex and Touchdown PCR for Microsatellite Analysis -- Detection of Microsatellite Instability and Loss of Heterozygosity Using DNA Extracted from Formalin-Fixed Paraffin-Embedded Tumor Material by Fluorescence-Based Multiplex Microsatellite PCR -- Reduction of Shadow Band Synthesis During PCR Amplification of Repetitive Sequences from Modern and Ancient DNA -- Degenerate Oligonucleotide-Primed PCR -- Mutation Detection Using RT-PCR-RFLP -- Multiplex Amplification Refractory Mutation System for the Detection of Prothrombotic Polymorphisms -- PCR-SSCP Analysis of Polymorphism -- PCR-Based Sequencing -- Sequencing -- Preparation and Direct Automated Cycle Sequencing of PCR Products -- Nonradioactive PCR Sequencing Using Digoxigenin -- Direct Sequencing by Thermal Asymmetric PCR -- Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing --
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|a Creation of Chimeric Junctions, Deletions, and Insertions by PCR -- Recombination and Site-Directed Mutagenesis Using Recombination PCR -- Megaprimer PCR.
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|a Direct Sequencing with Highly Degenerate and Inosine-Containing Primers -- Determination of Unknown Genomic Sequences Without Cloning -- Cloning PCR Products for Sequencing in M13 Vectors -- DNA Rescue by the Vectorette Method -- Technical Notes for Sequencing Difficult Templates -- In Situ PCR and Prins -- PCR-Based Detection of Nucleic Acids in Chromosomes, Cells, and Tissues -- Cycling Primed In Situ Amplification -- Direct and Indirect In Situ PCR -- Reverse Transcriptase In Situ PCR -- Primed In Situ Nucleic Acid Labeling Combined with Immunocytochemistry to Simultaneously Localize DNA and Proteins in Cells and Chromosomes -- Cloning and Mutagenesis -- Cloning and Mutagenesis -- Using T4 DNA Polymerase to Generate Clonable PCR Products -- A T-Linker Strategy for Modification and Directional Cloning of PCR Products -- Cloning Gene Family Members Using PCR with Degenerate Oligonucleotide Primers -- cDNA Libraries from a Low Amount of Cells --
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| 653 |
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|a Cell Biology
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| 653 |
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|a Cytology
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| 700 |
1 |
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|a Stirling, David
|e [editor]
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| 041 |
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7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SPRPROT
|a Springer Protocols Archive 1981-2004
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| 490 |
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|a Methods in Molecular Biology
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| 024 |
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|a 10.1385/1592593844
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| 856 |
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|u https://doi.org/10.1385/1592593844?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 571.6
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| 520 |
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|a Drawing on the proven qualities of the much praised and widely used first edition, John M. S. Bartlett and David Stirling have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These successful methods include real-time PCR, SNP analysis, nested PCR, direct PCR, and long-range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. In situ PCR methods and their application in parallel with other methods, such as immunohistochemistry, are also included. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. Cutting-edge and highly practical, PCR Protocols, Second Edition provides both novice and experienced investigators with an up-to-date compendium of powerful PCR methods for easy reference and consultation in the day-to-day performance of PCR-based experimentation, one that will enhance understanding of PCR, satisfy current needs, and point to powerful future applications
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