Neuropeptide Protocols
After 20 years of intensive effort, novel neuropeptides continue to be discovered, and the field of neuropeptide research is still expanding. As new analytical techniques become available, their applicability to the study of neu ropeptides brings fresh insights into the properties and functions of...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
1997, 1997
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| Edition: | 1st ed. 1997 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Preparation of Neuropeptide-Containing Fractions from Biological Materials
- Purification of Extracted Peptides for Structural Analysis
- Amino Acid Sequencing of Neuropeptides
- Neuropeptide Gene Identification Using the Polymerase Chain Reaction
- Solid-Phase Synthesis of Neuropeptides by Fmoc Strategies
- Incorporation of Stable Pseudopeptide Bonds
- Synthesis of Conformationally Restricted Peptides
- Purification of Synthetic Peptides by High Performance Liquid Chromatography
- Molecular Weight Estimation for Neuropeptides Using Size-Exclusion High Performance Liquid Chromatography
- Molecular Weight Determinations Using Polyacrylamide Gel Electrophoresis with Tris-Tricine Buffers
- Determination of Neuropeptides by Capillary Electrophoresis
- Characterization of Neuropeptide Processing by Fast Atom Bombardment Mass Spectrometry
- Analysis of Neuropeptides by Size-Exclusion HPLC Linked to Electrospray Ionization Mass Spectrometry
- Preparation of a Membrane Fraction for Receptor Studies and Solubilization of Receptor Proteins with Retention of Biological Activity
- Radioligand Binding Using 125Labeled Peptides
- Analysis of Data from “Cold Saturation” Radioligand Binding Experiments
- Organ/Tissue Preparations for the Assessment of Agonist/Antagonist Activity
- Measurement of Efflux Rates from Brain to Blood
- Assay of Neuropeptidases Using Fluorogenic Substrates
- Characterization of Neuropeptidases Using Inhibitors
- Identification of Peptides by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) and Direct Analysis of the Laterobuccal Nerve from the Pond Snail Lymnaea stagnalis
- Use of Circular Dichroism to Determine Secondary Structure of Neuropeptides
- 1H Nuclear Magnetic Resonance (NMR) in the Elucidation of Peptide Structure
- The Study of Membrane- or Receptor-Bound Neuropeptides by NMR
- Molecular Modeling of Neuropeptides
- Tritium Labeling of Neuropeptides
- The Use of IODO-GEN for Preparing 125I-Labeled Peptides and Their Purification by Reversed-Phase High Performance Liquid Chromatography
- Production of Antisera Using Peptide Conjugates
- Radioimmunoassay
- Enzyme-Linked Immunosorbent Assay of Peptides
- Sample Preparation for Peptide Immunocytochemistry
- lmmunocytochemical Methods for Regulatory Peptides
- Ultrastructural Localization of Peptides Using Immunogold Labeling