Immobilization of Enzymes and Cells

Immobihzatron of enzymes, cells, and organelles has expanded greatly in the past 30 years as the advantages of immobilization have been evaluated and utilized in analyttcal, biotransformation, and medical applications. A c- sequence of this explosion of technology IS that there is now a bewildering...

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Bibliographic Details
Other Authors: Bickerstaff, Gordon (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 1997, 1997
Edition:1st ed. 1997
Series:Methods in Biotechnology
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • Immobilization of Enzymes and Cells
  • Immobilization of Enzymes by Selective Adsorption on Biotinylaminopropyl Celite or Glass
  • Immobilization of Proteins on Thionyl Chloride-Activated Controlled-Pore Glass
  • Enzyme Immobilization on Nylon
  • Visual Assessment of Enzyme Immobilization
  • Immobilization in Carrageenans
  • Entrapment in Calcium Alginate
  • Entrapment of Enzymes and Cells in Poly (2-Hydroxyethyl Methacrylate) Supports
  • Activation of Rayon/Polyester Cloth for Protein Immobilization
  • Immobilization of Enzymes on Microelectrodes Using Chemical Crosslinking
  • Photolithographic Patterning of Enzyme Membranes for the Modification of Microelectrodes
  • Electrochemical-Based Immobilization of Enzymes
  • Immobilization of Enzymes on Thermo-Responsive Polymers
  • Immobilization of Photosynthetic Membranes in an Albumin-Glutaraldehyde Crosslinked Matrix
  • Poly(Ethylene Glycol) Crosslinked to Albumin as a Support for Enzyme Immobilization
  • Poly(Carbamoyl Sulfonate) Hydrogels
  • Enzyme Immobilization on Polyethyleneimine-Coated Magnetite Particles
  • Immobilization of Enzymes and Proteins on Red Blood Cells
  • Cellulose Paper Support for Immobilization
  • Immobilization of Cells Using Electrostatic Droplet Generation
  • Hepatocyte Immobilization in Agarose and Functional Integrity Testing
  • Immobilized Hepatocytes in Xenobiotic Biotransformation Studies
  • Immobilization of Liposomes and Proteoliposomes in Gel Beads
  • Cell Immobilization with Phosphorylated Polyvinyl Alcohol (PVA) Gel
  • Covalent Immobilization of Enzymes to Graphitic Particles
  • Enzyme Immobilization Using Chitosan-Xanthan Complexes
  • Calcium Alginate Film Formed on a Stainless Steel Mesh
  • Preparation of Immobilized Subunits of a Multisubunit Enzyme
  • Characterization of Enzyme Activity, Protein Content, and Thiol Groups in Immobilized Enzymes
  • Immobilization of Enzymes Acting on Macromolecular Substrates
  • Immobilization of Enzymes on Glyoxyl Agarose
  • Stabilization of Immobilized Enzymes by Chemical Modification with Polyfunctional Macromolecules
  • Covalent Immobilization of Enzymes Using Commercially Available CDI-Activated Agarose
  • Immobilization of Cells in Polyelectrolyte Complexes
  • Coimmobilization of Enzymes and Cells
  • Adsorption of Lipase on Inorganic Supports
  • Immobilization of Enzymes on Inorganic Supports by Covalent Methods
  • Use of Divalent Metal Ions Chelated to Agarose Derivatives for Reversible Immobilization of Proteins
  • Transition Metal Methods for Immobilization of Enzymes and Cells