New Nucleic Acid Techniques
| Other Authors: | |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
1988, 1988
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| Edition: | 1st ed. 1988 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Crosslinking of Single-Stranded DNA to Resins
- Extraction of RNA from Plants
- Isolation of Plant Nuclei
- In Vitro Transcription in Plant Nuclei
- Large-Scale Isolation of Ti Plasmid DNA
- Transformation and Regeneration of Dicotyledonous Plants
- Plant Protoplast Fusion
- Plant Tissue Culture
- Direct Gene Transfer into Plant Protoplasts
- Isolation of Wheat Chloroplasts and Chloroplast DNA
- DNA Labeling by Isolated Wheat Chloroplasts
- Construction of Mammalian Genomic Libraries Using ? Replacement Vectors
- Cosmid Library Construction
- Construction of cDNA Libraries in ?gt10 or ?gt11
- Immunoscreening of ?gt11 Expression Libraries Using an Avidin–Biotin Detection System
- Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides
- Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors
- Expression Screening of cDNA Libraries in pEX
- Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins
- Epitope Mapping Using pEX
- DNA Transfection of Mammalian Cells Using Polybrene
- Expression of Foreign Genes in Mammalian Cells
- Dot-Blot Hybridization Method
- Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes
- Photobiotin-Labeled DNA and RNA Hybridization Probes
- Enzyme-Labeled DNA Probes
- Luminescent Detection of Specific DNA Sequences
- Detection of DNA Sequences Using Biotinylated Probes
- Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde
- Northern Blotting
- Hybrid-Release Translation
- Hybrid-Arrested Translation
- In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells
- Use of Elutips to Purify DNA
- Plasmid Preparation on Sephacryl S1000
- Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels
- Use of 35S Nucleotides for DNA Sequencing
- Direct Dideoxy DNA Sequencing
- Computer Applications to Molecular Biology
- Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique
- Oligonucleotide Synthesis Using the Manual Phosphotriester Method
- Manual Oligonucleotide Synthesis Using the Phosphoramidite Method
- Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis
- Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries